右旋糖酐測定色譜應用方法

Dextran Ladder Standard in HILIC with Fluorescence Detection

GU1 - GU15: 2-aminobenzamide (2-AB) labelled glucose homopolymers with 1 - 15 glucose monomer units

CONDITIONS
Column:ACE Excel 3 Glycan
Dimensions:150 x 2.1 mm
Mobile Phases:

A: 100 mM Ammonium formate in H2O (pH 4.5)
B: MeCN

Flow Rate: 0.5 mL/min

Injection: 1.5 μL

Temperature: 55 ℃

Detection: FLD, Aex = 260 nm, Aem = 430 nm

Instrument: VWR Chromaster with FLD

Dwell Volume: 1950 μL

方法供貨信息：

Product	描述	規格	貨號
ACE Excel 3 Glycan	UHPLC column	150 x 2.1 mm	EXL-1116-1502
Acetonitrile	VWR CHROMANORM® gradient grade	2.5 L	83639.320
Water	VWR CHROMANORM® gradient grade	2.5 L	83650.320
Ammonium formate	VWR CHROMANORM® for LC-MS	100 g	84884.180
VWR Chromaster HPLC system	Pre-configured complete system with UV detector		903-0337
VWR Fluorescence detector	Chromaster HPLC 5440 fluorescence detector		903-0527
UHPLC guard cartridge	For 2.1 mm i.d. columns	3 pk	EXL-1P16-GD2U
UHPLC guard holder	Required for use of UH PLC guard cartridges	1	H0011

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